Universitätsklinikum des Saarlandes und Medizinische Fakultät der Universität des Saarlandes
Klinik für Strahlentherapie und Radioonkologie
Leitung: Univ. Prof. Dr. med. M. Hecht
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Hier finden Sie Publikationen zum BMBF-Verbundprojekt:


    AP1: Reparatur-Foci als Marker einer chronischen Strahlenexposition

    • Subedi P, Gomolka M, Moertl S, Dietz A. Ionizing Radiation Protein Biomarkers in Normal Tissue and Their Correlation to Radiosensitivity: A Systematic Review. J Pers Med. 2021 Feb 19;11(2):140. PMID: 33669522
    • Bucher M, Endesfelder D, Roessler U, Borkhardt A, Dückers G, Kirlum HJ, Lankisch P, Oommen PT, Niehues T, Rübe CE, Baumgartner I, Bunk F, Moertl S, Hornhardt S, Gomolka M. Analysis of chromosomal aberrations and γH2A.X foci to identify radiation-sensitive ataxia-telangiectasia patients. Mutat Res. 2021 Jan-Feb;861-862:503301. PMID: 33551102
    • Dietz A, Gomolka M, Mörtl S, Subedi P. Ionizing radiation protein biomarkers in normal tissue and their correlation to radiosensitivity: protocol for a systematic review. J. Pers. Med. 2020 Dec; 11(1), 3; PMID: 33375047
    • Bucher M, Duchrow L, Endesfelder D, Roessler U, Gomolka M. Comparison of inexperienced operators and experts in γH2A.X and 53BP1 foci assay for high-throughput biodosimetry approaches in a mass casualty incident. Int J Radiat Biol. 2020 Oct;96(10):1263-1273. PMID: 32673132
    • Gomolka M & Bucher M. Die Deutsche Uranbergarbeiter Biobank. Management & Krankenhaus, 11/2019
    • Gürtler A, Hauptmann M, Pautz S, Kulka U, Friedl AA, Lehr S, Hornhardt S, Gomolka M. The inter-individual variability outperforms the intra-individual variability of differentially expressed proteins prior and post irradiation in lymphoblastoid cell lines. Arch Physiol Biochem. 2014 Dec;120(5):198-207. PMID: 25174346
    • Hornhardt S, Rößler U, Sauter W, Rosenberger A, Illig T, Bickeböller H, Wichmann HE, Gomolka M. Genetic factors in individual radiation sensitivity. DNA Repair (Amst). 2014 Apr;16:54-65. PMID: 24674628


    AP2: Akkumulation von RF nach Niedrig-Dosis-Bestrahlung

    • Schmal Z, Hammer B, Müller A, Rübe CE.Fractionated low-dose radiation induces long-lasting inflammatory responses in the hippocampus. Int J Radiat Oncol Biol Phys. 2021, under review
    • Rübe CE, Bäumert C, Schuler N, Isermann A, Schmal Z, Glanemann M, Mann C, Scherthan H. Human skin aging is associated with increased expression of the histone variant H2A.J in the epidermis. NPJ Aging Mech Dis. 2021 Apr 1;7(1):7. PMID: 33795696
    • Schmal Z, Isermann A, Hladik D, von Toerne C, Tapio S, Rübe CE. DNA damage accumulation during repeated radiation exposures compromises hippocampal neurogenesis. Radiother Oncol. 2019 Aug;137:45-54. PMID: 31063923
    • Schuler N, Timm S, Rübe CE. Hair follicle stem cell fate is dependent on chromatin remodeling capacity following low-dose radiation. Stem Cells. 2018 Apr;36(4):574-588. PMID: 29282803
    • Contrepois K, Coudereau C, Benayoun BA, Schuler N, Roux PF, Bischof O, Courbeyrette R, Carvalho C, Thuret JY, Ma Z, Derbois C, Nevers MC, Volland H, Redon CE, Bonner WM, Deleuze JF, Wiel C, Bernard D, Snyder MP, Rübe CE, Olaso R, Fenaille F, Mann C. Histone variant H2A.J accumulates in senescent cells and promotes inflammatory gene expression. Nat Commun. 2017 May 10;8: 14995. PMID: 28489069
    • Lorat Y, Schanz S., Rübe CE. Ultrastructural insights into the biological significance of persisting DNA damage foci after low doses of ionizing radiation. Clin Cancer Res. 2016 Nov 1;22(21):5300-5311. PMID: 27199493
    • Grewenig A, Schuler N, Rübe CE. Persistent DNA damage in spermatogonial stem cells after fractionated low-dose irradiation of testicular tissue. Int J Radiat Oncol Biol Phys 2015; 92 (5): 1123-1131. PMID: 26059351
    • Flockerzi E, Schanz S, Rübe CE. Even low doses of radiation lead to DNA damage accumulation in lung tissue according to the genetically-defined DNA repair capacity. Radiother Oncol. 2014 May;111(2):212-8. PMID: 24746565


    AP3: RF als Marker der individuellen Strahlenempfindlichkeit

    • Bakr A, Siegmund M, Lorat Y, Elsesy ME, Rieckmann T, Meien S, Oing C, Rübe CE, Petersen C, Rothkamm K, Mansour WY. A novel role for RAP80 in DNA double strand break end resection through targeting EXO1 to proteasomal degradation. To be submitted
    • Sanchez-Cano C, Alvarez-Puebla RA, Abendroth JM, Beck T, Blick R, Cao Y, Caruso F, Chakraborty I, Chapman HN, Chen C, Cohen BE, Conceição ALC, Cormode DP, Cui D, Dawson KA, Falkenberg G, Fan C, Feliu N, Gao M, Gargioni E, Glüer CC, Grüner F, Hassan M, Hu Y, Huang Y, Huber S, Huse N, Kang Y, Khademhosseini A, Keller TF, Körnig C, Kotov NA, Koziej D, Liang XJ, Liu B, Liu S, Liu Y, Liu Z, Liz-Marzán LM, Ma X, Machicote A, Maison W, Mancuso AP, Megahed S, Nickel B, Otto F, Palencia C, Pascarelli S, Pearson A, Peñate-Medina O, Qi B, Rädler J, Richardson JJ, Rosenhahn A, Rothkamm K, Rübhausen M, Sanyal MK, Schaak RE, Schlemmer HP, Schmidt M, Schmutzler O, Schotten T, Schulz F, Sood AK, Spiers KM, Staufer T, Stemer DM, Stierle A, Sun X, Tsakanova G, Weiss PS, Weller H, Westermeier F, Xu M, Yan H, Zeng Y, Zhao Y, Zhao Y, Zhu D, Zhu Y, Parak WJ. X-ray-Based Techniques to Study the Nano-Bio Interface. ACS Nano. 2021 Mar 23;15(3):3754-3807. PMID: 33650433
    • Köcher S, Volquardsen J, Perugachi Heinsohn A, Petersen C, Roggenbuck D, Rothkamm K, Mansour WY. Fully automated counting of DNA damage foci in tumor cell culture: A matter of cell separation. DNA Repair (Amst). 2021 Mar 16;102:103100. PMID: 33812230
    • Degenhardt S, Dreffke K, Schötz U, Petersen C, Engenhart-Cabillic R, Rothkamm K, Dahm-Daphi J, Dikomey E, Mansour WY. Establishment of a transformation coupled in vitro end joining assay to estimate radiosensitivity in tumor cells. Frontiers in Oncology, 2020 Aug;10(1480). PMID: 32974177
    • Elsesy ME, Oh-Hohenhorst SJ, Löser A, Oing C, Mutiara S, Köcher S, Meien S, Zielinski A, Burdak-Rothkamm S, Tilki D, Hartwig H, Schwarz R, Petersen C Bokemeyer C, Rothkamm K, Mansour WY. Second-generation antiandrogen therapy radiosensitizes prostate cancer regardless of castration state through inhibition of DNA double strand break repair. Cancers (Basel). 2020 Aug 31;12(9):E2467. PMID: 32878283
    • Burdak-Rothkamm S, Mansour WY, Rothkamm K. DNA Damage Repair Deficiency in Prostate Cancer. Trends Cancer. 2020 Nov;6(11):974-984. Epub 2020 Jun 6. PMID: 32517958
    • Köcher S, Beyer B, Lange T, Nordquist L, Volquardsen J, Burdak-Rothkamm S, Schlomm T, Petersen C, Rothkamm K, Mansour WY. A functional ex vivo assay to detect PARP1-EJ repair and radiosensitization by PARP-inhibitor in prostate cancer. Int J Cancer. 2019 Apr 1;144(7):1685-1696. Epub 2019 Jan 5. PMID: 30478958
    • Mansour WY, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube-Magg C, Borgmann K, Simon R, Petersen C, Dikomey E, Rothkamm K. Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Sci Rep. 2018 Mar 2;8(1):3947. PMID: 29500400
    • Oing C, Tennstedt P, Simon R, Volquardsen J, Borgmann K, Bokemeyer C, Petersen C, Dikomey E, Rothkamm K, Mansour WY. BCL2-overexpressing prostate cancer cells rely on PARP1-dependent end-joining and are sensitive to combined PARP inhibitor and radiation therapy. Cancer Lett. 2018 Jun 1;423:60-70.  Epub 2018 Mar 8. PMID: 29526801
    • Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, Rothkamm K, Mansour WY. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget 2016; 7:57679-57693. PMID: 27494840
    • Bakr A, Köcher S, Volquardsen J, Reimer R, Borgmann K, Dikomey E, Rothkamm K, Mansour WY. Functional crosstalk between DNA damage response proteins 53BP1 and BRCA1 regulates double strand break repair choice. Radiother Oncol 2016; 119:276-281. PMID: 26615718
    • Bakr A, Oing C, Köcher S, Borgmann K, Dornreiter I, Petersen C, Dikomey E, Mansour WY. Involvement of ATM in homologous recombination after end resection and RAD51 nucleofilament formation. Nucleic Acids Res 2015; 43:3154-3166. PMID: 25753674


    AP4: Akkumulierte RF als Marker des Normalgeweberisikos

      • Lorat Y, Reindl J, Isermann A, Rübe C, Friedl A, Dollinger G, Rübe CE. Focused ion microbeam irradiation induces clustering of DNA double-strand breaks in heterochromatin visualized by nanoscale-resolution electron microscopy. Int J Mol Sci. 2021, under review
      • Isermann A, Mann C, Rübe CE. Histone Variant H2A.J Marks Persistent DNA Damage and Triggers the Secretory Phenotype in Radiation-Induced Senescence .Int. J. Mol. Sci. 2020, 21(23), 9130. PMID: 33266246
      • Mangelinck A, Coudereau C, Courbeyrette R, Ourarhni K, Hamiche A, Redon C, Bonner WM, van Dijk E, Derbois C, Olaso R, Deleuze JF, Fenaille F, Rübe CE, Thuret JY, Mann C. The H2A.J histone variant contributes to Interferon-Stimulated Gene expression in senescence by its weak interaction with H1 and the derepression of repeated DNA sequences. bioRxiv preprint doi: https://doi.org/10.1101/2020.10.29.361204; posted October 30, 2020
      • Lorat Y, Fleckenstein J, Görlinger P, Rübe C, Rübe CE. Assessment of DNA damage by 53PB1 and pKu70 detection in peripheral blood lymphocytes by immunofluorescence and high-resolution transmission electron microscopy.  Strahlenther Onkol. 2020 Sep;196(9):821-833. PMID: 32006067
      • Timm S, Lorat Y, Jakob B, Taucher-Scholz G, Rübe CE. Clustered DNA damage concentrated in particle trajectories causes persistent large-scale rearrangements in chromatin architecture.  Radiother Oncol. 2018 Dec;129(3):600-610. Radiother Oncol. 2018. PMID: 30049456
      • Schuler N, Palm J, Schmitz S, Lorat Y, Rübe CE. Increasing genomic instability during cancer therapy in a patient with Li-Fraumeni syndrome.  Clinical & Translational Radiation Oncology 2017, 7:71-78. PMID: 29594232
      • Lorat Y, Timm S, Jakob B, Taucher-Scholz G, Rübe CE. Clustered double-strand breaks in heterochromatin perturb DNA repair after high linear energy transfer irradiation.  Radiother Oncol. 2016 Oct;121(1):154-161. PMID: 27637859
      • Lorat Y, Brunner CU, Schanz S, Jakob B, Taucher-Scholz G, Rübe CE. Nanoscale analysis of clustered DNA damage after high-LET irradiation by quantitative electron microscopy--the heavy burden to repair.  DNA Repair (Amst). 2015 Apr;28:93-106. PMID: 25659339


        AP5: RF als Marker der Tumorstrahlenempfindlichkeit

        • Suckert T, Rassamegevanon T, Müller J, Dietrich A, Graja A, Reiche M, Löck S, Krause M, Beyreuther E, von Neubeck C. Applying Tissue Slice Culture in Cancer Research-Insights from Preclinical Proton Radiotherapy. Cancers (Basel). 2020 Jun 16;12(6):1589. PMID: 32560230
        • Rassamegevanon T, Löck S, Baumann M, Krause M, von Neubeck C. Comparable radiation response of ex vivo and in vivo irradiated tumor samples determined by residual γH2AX. Radiother Oncol. 2019 Oct;139:94-100. PMID: 31445839
        • Meneceur S, Löck S, Gudziol V, Hering S, Bütof R, Rehm M, Baumann M, Krause M, von Neubeck C. Residual gammaH2AX foci in head and neck squamous cell carcinomas as predictors for tumour radiosensitivity: Evaluation in pre-clinical xenograft models and clinical specimens. Radiother Oncol. 2019 Aug;137:24-31. PMID: 31048234
        • Rassamegevanon T, Löck S, Baumann M, Krause M, von Neubeck C. Heterogeneity of γH2AX Foci Increases in Ex Vivo Biopsies Relative to In Vivo Tumors. Int J Mol Sci. 2018 Sep 4;19(9):2616. PMID: 30181446
        • Rassamegevanon T, Löck S, Range U, Krause M, Baumann M, von Neubeck C. Tumor heterogeneity determined with a γH2AX foci assay: A study in human head and neck squamous cell carcinoma (hHNSCC) models.  Radiother Oncol. 2017 Sep;124(3):379-385. PMID: 28739384
        • Menegakis A, von Neubeck C, Yaromina A, Thames H, Hering S, Hennenlotter J, Scharpf M, Noell S, Krause M, Zips D, Baumann M. γH2AX assay in ex vivo irradiated tumour specimens: A novel method to determine tumour radiation sensitivity in patient-derived material. Radiother Oncol. 2015 Sep;116(3):473-9. PMID: 25866027
        • Menegakis A, De Colle C, Yaromina A, Hennenlotter J, Stenzl A, Scharpf M, Fend F, Noell S, Tatagiba M, Brucker S, Wallwiener D, Boeke S, Ricardi U, Baumann M, Zips D. Residual γH2AX foci after ex vivo irradiation of patient samples with known tumour-type specific differences in radio-responsiveness.  Radiother Oncol. 2015 Sep;116(3):480-5. PMID: 26297183

        AP6: RF als Marker einer genomischen Instabilität

        • Al Zubaidi T, Gehrisch OHF, Genois MM, Liu Q, Lu S, Kung J, Xie Y, Schuemann J, Lu HM, Hata AN, Zou L, Borgmann K, Willers H. Targeting the DNA replication stress phenotype of KRAS mutant cancer cells. Sci Rep. 2021 Feb 11;11(1):3656. PMID: 33574444
        • Bold IT, Specht AK, Droste CF, Zielinski A, Meyer F, Clauditz TS, Münscher A, Werner S, Rothkamm K, Petersen C, Borgmann K. DNA Damage Response during Replication Correlates with CIN70 Score and Determines Survival in HNSCC Patients. Cancers (Basel). 2021 Mar 10;13(6):1194. PMID: 33801877
        • Mognato M, Burdak-Rothkamm S, Rothkamm K. Interplay between DNA replication stress, chromatin dynamics and DNA-damage response for the maintenance of genome stability. Mutation Research/Reviews in Mutation Research, 787, 2021. https://doi.org/10.1016/j.mrrev.2020.108346
        • Meyer F, Engel AM, Krause AK, Wagner T, Poole P, Peitzsch C, Dubrovska A, Rothkamm K, Petersen C, Borgmann K. Avoidance of radiation-induced DNA replication stress results in lower immunogenic, cytosolic DNA by upregulated homologous recombination in breast cancer stem cells. Submitted to Cancer letters 2020
        • Rieckhoff J, Meyer F, Classen S, Zielinski A, Riepen B, Wikman H, Petersen C, Rothkamm K, Borgmann K, Parplys AC. Exploiting Chromosomal Instability of PTEN-Deficient Triple-Negative Breast Cancer Cell Lines for the Sensitization against PARP1 Inhibition in a Replication-Dependent Manner. Cancers (Basel). 2020 Sep 29;12(10):2809. PMID: 33003585
        • Meyer F, Becker S, Classen S, Paprlys AC, Mansour WY, Riepen B, Timm S, Ruebe C, Jasin M, Wikman H, Petersen C, Rothkamm K, Borgmann K. Prevention of DNA Replication Stress by CHK1 Leads to  Chemoresistance Despite a DNA Repair Defect in Homologous Recombination in Breast Cancer. In: Cells, 2020 Jan 17;9(1). PMID: 31963582
        • Schulz A, Meyer F, Dubrovska A, Borgmann K. Cancer Stem Cells and Radioresistance:  DNA Repair and Beyond. Cells. 2020 Jan 17;9(1):238. PMID: 31234336