Saarland University Faculty of Medicine
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Methods and Technologies

Beside expertise in the fields of molecular biology, biochemistry and cell biology, the research groups participating in the graduate school provide a large variety of methods and strategies to study Ca2+-signals and their functional consequences with high temporal and spatial resolution:

 

 

 

  • Measurement and control of intracellular Ca2+-signals: high resolution calcium-imaging and ultra-fast UV-flash photolysis of caged compounds

 

  • Imaging techniques: Bioluminescence resonance energy transfer, laser scanning and real-time confocal microscopy, 2-photon microscopy, Time-Lapse video microscopy, Total-Internal-Reflection-Fluorecence microscopy (TIRF).

 

  • Electrophysiology and measurements of secretion at the level of single cells and single vesicles: Patch clamp technique, high-resolution membrane capacitance measurements, carbon fiber amperometry, electrical measurements of exocytotic fusion pores, elektroenzephalo- und electrocorticograms.

 

  • Analyses of cellular ultrastructure: Transmission- und Scanning electron microscopy

 

  • Protein-Protein-Interactions in-vitro and in-vivo: Biochemical methods, Multi-angle light scattering (MALS), Plasmon-resonance-transfer (BIAcore), Yeast-Two Hybrid system, Fluorescence energy transfer (FRET)

 

  • Techniques for purification of recombinant and native proteins

 

  • Protein structure analyses: X-ray cristallography

 

  • Viral vector constructs and siRNA-technology: Gene transfer with Semliki-Forest, Adeno- und Lenti-Viruses into primary cells

 

  • Quantitative single cell PCR

 

  • Transgenic mouse technology

 

  • Flow-through-cytometry & Fluorescent Associated Cell Sorting (FACS)

 

  • Specific cell cultures: Primary cultures of neurons, chromaffin cells, endothelial cells, mast cells, cardiomyocytes, pancreatic beta-cells, T-lymphocytes, organotypic cultures of retina

 

  • Pharmacological tests, receptor binding studies

 

  • Mouse behavioral testing